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| CHIM0627-1 | Structure of biological macromolecules (experimental aspects) : part b1 (RX, NMR)
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| Duration : | 15h Th, 10h Pr |
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| Number of credits : |
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| Lecturer : | Paulette Charlier, Christian Damblon |
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| Coordinator : | Paulette Charlier |
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Language(s) of instruction :
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| French language |
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Organisation and examination :
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| Teaching in the second semester |
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Course contents :
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| The course goes into deeper description of of the techniques used to investigate the structure of proteins: X-ray Crystallography and Nuclear Magnetic Resonance.
I- X-ray diffraction (P. Charlier)
Theoretical part: 1- High resolution structure, 2- Neutron diffraction, 3- Laue diffraction
Practical part: Solving of a protein structure in silico on the basis of X-ray diffraction data.
II- Nuclear Magnetic Resonance (C. Damblon)
Theoretical part: 1- Protein-ligand interactions
Practical part: Data collection with a 500MHz spectrometer. |
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Learning outcomes of the course :
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| This course gives to the future bioinformaticians the bases necessary to understand the principles of determination of 3D protein structure by X-ray crystallography and nuclear magnetic resonance in order to enable them to make an objective use of the models. It gives them an outline of the capacities and limits of various techniques. |
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Prerequisites and co-requisites/ Recommended optional programme components :
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| Course of physics of 1st cycle. Basic knowledge in X-ray crystallography. Good knowledge of the basic principles of the structures of proteins. Biophysical course. |
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Planned learning activities and teaching methods :
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| Tutorials dedicated to the Pymol software use: obseravtion and analysis of macromolecules structures. Use of PDB database.
Solving of a protein structure in silico on the basis of X-ray diffraction data. |
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Mode of delivery (face-to-face ; distance-learning) :
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| To be decided in concertation with the different lecturers. |
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Recommended or required readings :
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| The PowerPoint presentations of the theoretical course will be available on CD as well as the notes of practical works.
Books recommended:
- "Crystallography made hook clear: With guide for users for macromolecular models."
Scale Rhodos, Academic Press 2000
- "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999
CD roms:
- PS ² and Kinemage Supplement to "Introduction to Protein Structure" Carl Branden and
John Tooze, Garland Publishing 1999 |
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Assessment methods and criteria :
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| To be decided in concertation with the different lecturers. Most probably on the basis of a research article. |
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Training(s) :
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Organizational remarks :
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Contacts :
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| Paulette Charlier, PhD, Chargé de CoursCristallographie des macromolécules biologiques, Centre d'Ingénierie des Protéines, Département des Sciences de la Vie, Université de Liège, Institut de Physique, B5a, B-4000 Liège, Belgium
Tel: +32 (0)4 366 36 19
Fax: +32 (0)4 366 49 54
E-mail : Paulette.Charlier@ulg.ac.be
http://www.cip.ulg.ac.be/
DAMBLON Christian
Département de chimie (sciences) / Chimie biologique structurale
Bât. B6 Chimie biologique structurale
allée du 6 Août 3
4000 Liège 1
Belgique
Tél. ULg +32 4 3663788
Fax ULg +32 4 3664577
c.damblon@ulg.ac.be |
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