University of Liege | Version française
Academic year 2014-2015Value date : 19/12/2014
CHIM0627-1  Structure of biological macromolecules (experimental aspects) : part b1 (RX, NMR)

Duration :  15h Th, 10h Pr
Number of credits :  
Master in Bio-informatics and Modelling, Research focus, 2nd year3
Lecturer :  Paulette Charlier, Christian Damblon
Coordinator :  Paulette Charlier
Language(s) of instruction :  
French language
Organisation and examination :  
Teaching in the first semester, review in January
Course contents :  
The course goes into deeper description of of the techniques used to investigate the structure of proteins: X-ray Crystallography and Nuclear Magnetic Resonance.

I- X-ray diffraction (P. Charlier) Theoretical part: 1- High resolution structure, 2- Neutron diffraction, 3- Laue diffraction Practical part: Solving of a protein structure in silico on the basis of X-ray diffraction data.
II- Nuclear Magnetic Resonance (C. Damblon) Theoretical part: 1- Protein-ligand interactions Practical part: Data collection with a 500MHz spectrometer.
Learning outcomes of the course :  
This course gives to the future bioinformaticians the bases necessary to understand the principles of determination of 3D protein structure by X-ray crystallography and nuclear magnetic resonance in order to enable them to make an objective use of the models. It gives them an outline of the capacities and limits of various techniques.
Prerequisites and co-requisites/ Recommended optional programme components :  
Course of physics of 1st cycle. Basic knowledge in X-ray crystallography. Good knowledge of the basic principles of the structures of proteins. Biophysical course.
Planned learning activities and teaching methods :  
Tutorials dedicated to the Pymol software use: obseravtion and analysis of macromolecules structures. Use of PDB database. Solving of a protein structure in silico on the basis of X-ray diffraction data.
Mode of delivery (face-to-face ; distance-learning) :  
To be decided in concertation with the different lecturers.
Recommended or required readings :  
The PowerPoint presentations of the theoretical course will be available on CD as well as the notes of practical works. Books recommended: - "Crystallography made hook clear: With guide for users for macromolecular models." Scale Rhodos, Academic Press 2000 - "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999 CD roms: - PS ² and Kinemage Supplement to "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999
Assessment methods and criteria :  
To be decided in concertation with the different lecturers. Most probably on the basis of a research article.
Work placement(s) :  
Organizational remarks :  
Contacts :  
Paulette Charlier, PhD, Chargé de CoursCristallographie des macromolécules biologiques, Centre d'Ingénierie des Protéines, Département des Sciences de la Vie, Université de Liège, Institut de Physique, B5a, B-4000 Liège, Belgium Tel: +32 (0)4 366 36 19 Fax: +32 (0)4 366 49 54 E-mail : Paulette.Charlier@ulg.ac.be http://www.cip.ulg.ac.be/
DAMBLON Christian Département de chimie (sciences) / Chimie biologique structurale Bât. B6 Chimie biologique structurale allée du 6 Août 3 4000 Liège 1 Belgique Tél. ULg +32 4 3663788 Fax ULg +32 4 3664577 c.damblon@ulg.ac.be



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